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Figure 1. Levels of sCD52 quantified by <t>ELISA.</t> (A) healthy blood donors (n ¼ 45) and patients with CLL (n ¼ 86). (B) patients with early CLL (n ¼ 44) and with progressive CLL (progressive CLL, n ¼ 42). (C) patients with early CLL and progressive CLL by Binet stage grading A (n ¼ 39 and n ¼ 6, early CLL and progressive CLL, respectively), vs. B/C (n ¼ 4 and n ¼ 36, early CLL and progres- sive CLL, respectively). (D) Levels of sCD52 and b2-microglobulin (b2m) in patients with early CLL (n ¼ 26) and progressive CLL (n ¼ 30). Statistical analyses were performed as unpaired Student’s t tests and linear regression analysis, data were logarithmically transformed.
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Figure 1. Levels of sCD52 quantified by ELISA. (A) healthy blood donors (n ¼ 45) and patients with CLL (n ¼ 86). (B) patients with early CLL (n ¼ 44) and with progressive CLL (progressive CLL, n ¼ 42). (C) patients with early CLL and progressive CLL by Binet stage grading A (n ¼ 39 and n ¼ 6, early CLL and progressive CLL, respectively), vs. B/C (n ¼ 4 and n ¼ 36, early CLL and progres- sive CLL, respectively). (D) Levels of sCD52 and b2-microglobulin (b2m) in patients with early CLL (n ¼ 26) and progressive CLL (n ¼ 30). Statistical analyses were performed as unpaired Student’s t tests and linear regression analysis, data were logarithmically transformed.

Journal: Leukemia & lymphoma

Article Title: Soluble CD52 is an indicator of disease activity in chronic lymphocytic leukemia.

doi: 10.1080/10428194.2017.1285027

Figure Lengend Snippet: Figure 1. Levels of sCD52 quantified by ELISA. (A) healthy blood donors (n ¼ 45) and patients with CLL (n ¼ 86). (B) patients with early CLL (n ¼ 44) and with progressive CLL (progressive CLL, n ¼ 42). (C) patients with early CLL and progressive CLL by Binet stage grading A (n ¼ 39 and n ¼ 6, early CLL and progressive CLL, respectively), vs. B/C (n ¼ 4 and n ¼ 36, early CLL and progres- sive CLL, respectively). (D) Levels of sCD52 and b2-microglobulin (b2m) in patients with early CLL (n ¼ 26) and progressive CLL (n ¼ 30). Statistical analyses were performed as unpaired Student’s t tests and linear regression analysis, data were logarithmically transformed.

Article Snippet: sCD52 was quantified in plasma or serum by a commercial CD52 ELISA kit (CSB-EL004943HU, Cusabio Biotech, China) following the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Transformation Assay

Figure 2. Levels of sCD52 and time to first treatment (TTFT) in ‘early’ CLL. (A) TTFT in patients with ‘early’ CLL divided by the median sCD52 level (n ¼ 44). (B) TTFT in patients with ‘early’ CLL at diagnosis based on sCD52 and IGHV mutational status, a sub- group of the patients shown in A (n ¼ 34). Statistical analyses were performed as log rank test, cumulative incidence rates are shown. M-IGHV: mutated IGHV, UM-IGHV: unmutated IGHV. The median level of sCD52 (274 ng/mL) in ‘early CLL’ patients with a sCD52 level above lower limit of quantification in the ELISA found to discriminate best between healthy donors and patients with CLL by ROC curve analysis, data not shown.

Journal: Leukemia & lymphoma

Article Title: Soluble CD52 is an indicator of disease activity in chronic lymphocytic leukemia.

doi: 10.1080/10428194.2017.1285027

Figure Lengend Snippet: Figure 2. Levels of sCD52 and time to first treatment (TTFT) in ‘early’ CLL. (A) TTFT in patients with ‘early’ CLL divided by the median sCD52 level (n ¼ 44). (B) TTFT in patients with ‘early’ CLL at diagnosis based on sCD52 and IGHV mutational status, a sub- group of the patients shown in A (n ¼ 34). Statistical analyses were performed as log rank test, cumulative incidence rates are shown. M-IGHV: mutated IGHV, UM-IGHV: unmutated IGHV. The median level of sCD52 (274 ng/mL) in ‘early CLL’ patients with a sCD52 level above lower limit of quantification in the ELISA found to discriminate best between healthy donors and patients with CLL by ROC curve analysis, data not shown.

Article Snippet: sCD52 was quantified in plasma or serum by a commercial CD52 ELISA kit (CSB-EL004943HU, Cusabio Biotech, China) following the manufacturer’s instructions.

Techniques: Biomarker Discovery, Enzyme-linked Immunosorbent Assay